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Swimming pools of pre-intended primers may help generate complete expression profiles, Specifically exactly where assay sensitivity is critically important.
For one particular, the CCI Benchrest primers ended up the flattest with the good deal, suggesting that they're no more challenging when compared to the Other folks and therefore are Possibly softer.
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30-06 examination hundreds display flattening—within healthier boundaries— and though it’s a small sample size determined by only one propellant sort, one bullet type, in one caliber/situation mix, I gleaned some fascinating observations.
Compared with oligo(dT) primers, these primers are not template particular and so are well suited for DNA synthesis using Klenow fragments with DNA templates or for cDNA synthesis making use of reverse transcriptase with mRNA templates.
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In distinction to random primers, oligo (dT) primers are not well suited for degraded samples but are exceptional for constructing cDNA libraries and advisable to be used with reverse transcriptases (RTs) as Superscript IV RT.
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